LOCUS pS_T7_sfGFP_r1 2452 bp DNA circular 10-JUL-2026 DEFINITION . ACCESSION SOURCE . ORGANISM . DNA circular UNIMARK pJS1 variant GGA features pJS1 variant GGA COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 1868..1886 /note="promoter for bacteriophage T7 RNA polymerase" /locus_tag="T7 promoter" /label="T7 promoter" /ApEinfo_label="T7 promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" RBS 1918..1940 /ugene_name="Unknown" /ugene_group="RBS" /locus_tag="strong RBS" /label="strong RBS" /ApEinfo_label="strong RBS" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" misc_feature 207..262 /locus_tag="EcoRi SacI KpnI SmaI PstI SphI HindIII MCS" /label="EcoRi SacI KpnI SmaI PstI SphI HindIII MCS" /ApEinfo_label="EcoRi SacI KpnI SmaI PstI SphI HindIII MCS" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" misc_feature 304..341 /locus_tag="MgApt" /label="MgApt" /ApEinfo_label="MgApt" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" terminator 366..413 /note="transcription terminator for bacteriophage T7 RNA polymerase" /locus_tag="T7 terminator" /label="T7 terminator" /ApEinfo_label="T7 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" misc_feature 424..453 /locus_tag="T500 terminator" /label="T500 terminator" /ApEinfo_label="T500 terminator" /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" CDS complement(471..1130) /gene="cat" /note="confers resistance to chloramphenicol" /product="chloramphenicol acetyltransferase" /translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFS SLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVA NMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA * /locus_tag="CmR" /label="CmR" /ApEinfo_label="CmR" /ApEinfo_fwdcolor="#ccffcc" /ApEinfo_revcolor="#ccffcc" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" rep_origin 1252..1840 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /locus_tag="ori" /label="ori" /ApEinfo_label="ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" CDS join(1948..2452,1..206) /ugene_name="Unknown" /ugene_group="CDS" /locus_tag="sfGFP-His" /label="sfGFP-His" /ApEinfo_label="sfGFP-His" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0" ORIGIN 1 ataacgttga agatggcagt gtgcagctgg cagatcatta tcagcagaat accccgattg 61 gtgatggtcc ggtgctgctg ccggataatc attatctgag cacgcagacc gttctgtcta 121 aagatccgaa cgaaaaacgg gaccacatgg ttctgcacga atatgtgaat gcggcaggta 181 ttacgcatca tcatcatcat cattaagaat tcgagctcgg tacccgggat cctctagagt 241 cgacctgcag gcatgcaagc ttgtcgaccg gctgctaaca aagcccgaaa ggaagctgag 301 ttgggatccc gactggcgag agccaggtaa cgaatggatc cgctgctgcc accgctgagc 361 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttggctagtc 421 gaccaaagcc cgccgaaagg cgggcttttc tgtctgaaag ccaattctga ttacgccccg 481 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 541 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 601 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa 661 tcaaaactgg tgaaactcac ccagggattg gctgacacga aaaacatatt ctcaataaac 721 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 781 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 841 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 901 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc 961 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga 1021 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga 1081 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat aacacccctt 1141 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt 1201 gcaatgtaac atcagagatt ttgagacaca acgtgagatc aaaggatctt cttgagatcc 1261 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 1321 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 1381 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 1441 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 1501 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 1561 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 1621 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 1681 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 1741 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 1801 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcgatccc 1861 gcgaaattaa tacgactcac tatagggaga ctacaacggt ttccctctag aaataatttt 1921 gtttaacttt aagaaggaga tatacatatg agcaaaggtg aagaactgtt taccggcgtt 1981 gtgccgattc tggtggaact ggatggcgat gtgaacggtc acaaattcag cgtgcgtggt 2041 gaaggtgaag gcgatgccac gattggcaaa ctgacgctga aatttatctg caccaccggc 2101 aaactgccgg tgccgtggcc gacgctggtg accaccctga cctatggcgt tcagtgtttt 2161 agtcgctatc cggatcacat gaaacgtcac gatttcttta aatctgcaat gccggaaggc 2221 tatgtgcagg aacgtacgat tagctttaaa gatgatggca aatataaaac gcgcgccgtt 2281 gtgaaatttg aaggcgatac cctggtgaac cgcattgaac tgaaaggcac ggattttaaa 2341 gaagatggca atatcctggg ccataaactg gaatacaact ttaatagcca taatgtttat 2401 attacggcgg ataaacagaa aaatggcatc aaagcgaatt ttaccgttcg cc //